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Functional characterization of pressurized liquid extracts of Spirulina platensis

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Abstract

Three different parameters (temperature, solvent, and extraction time) were studied regarding to pressure liquid extraction (PLE) of antioxidant and antimicrobial compounds from Spirulina platensis. Two different antioxidant methods, β-carotene bleaching method and DPPH• (2,2-Diphenyl-1-picrylhydrazyl hydrate) free radical scavenging assay, were used to determine the optimal PLE conditions for antioxidants extraction. The selected conditions were as follows: extraction temperature equal to 115 °C, extraction time equal to 15 min and ethanol as extracting solvent. The main antioxidant compounds found in this extract were identified as zeaxanthin, a myxoxanthophyll-like compound and very polar phenolic compounds. Moreover, antimicrobial activity of different PLE fractions was tested against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 11775, Candida albicans ATCC 60193, and Aspergillus niger ATCC 16404. Data obtained showed the hexane and petroleum ether extracts were slightly more active than ethanolic extracts. As for water extracts, none of them were active against the microorganisms tested. Data indicated that both 115 and 170 °C were the best extraction temperatures conditions in order to optimize the extraction of antimicrobial compounds, whereas 9 min was the optimal extraction time. Besides, C. albicans was the most sensitive microorganism to all Spirulina PLE extracts.

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References

  1. Chadwick R, Henson S, Moseley B, Koenen G, Liakopoulos M, Midden C, Palou A, Rechkemmer G, Schröder D, von Wright A (2003) Functional foods. Springer, Berlin, Heidelberg, New York, p 2

    Google Scholar 

  2. Dillon JC, Phuc AP, Dubacq JP (1995) World Rev Nutr Diet 77:32–46

    CAS  Google Scholar 

  3. Careri M, Furlattini L, Mangia A, Musci M, Anklam E, Theobald A, Holst C (2001) J Chromatogr A 912:61–71

    Article  CAS  Google Scholar 

  4. Mendes RL, Nobre BP, Cardoso MT, Pereira AP, Palabra AF (2003) Inorg Chim Acta 356:328–334

    Article  CAS  Google Scholar 

  5. Miranda MS, Cintra RG, Barros SBM, Manzini FJ (1998) Braz J Med Biol Res 31:1075–1079

    Article  CAS  Google Scholar 

  6. Piñero-Estrada JE, Bermejo-Bescós P, Villar-del Fresno AM (2001) Il Farmaco 59:497–500

    Article  Google Scholar 

  7. Subhashini J, Mahipal SVK, Reddy MC, Reddy MM, Rachamallu A, Reddanna P (2004) Biochem Pharmacol 68:453–462

    Article  CAS  Google Scholar 

  8. Hirahashi T, Matsumoto M, Hazeki K, Saeki Y, Ui M, Seya T (2002) Int Immunopharmacol 2:423–434

    Article  CAS  Google Scholar 

  9. Ozdemir G, Karabay NU, Dalay MC, Pazarbasi B (2004) Phytother Res 18:754–757

    Article  CAS  Google Scholar 

  10. Hayashi K, Hayashi T, Morita N (1993) Phytother Res 7:76–80

    Google Scholar 

  11. King JW (2000) Food Sci Technol Today 14:186–189

    Google Scholar 

  12. Denery JR, Dragull K, Tang CS, Li QX (2004) Anal Chim Acta 501:175–181

    Article  CAS  Google Scholar 

  13. Benthin B, Danz H, Hamburger M (1999) J Chromatogr A 837:211–219

    Article  CAS  Google Scholar 

  14. Piñeiro Z, Palma M, Barroso CG (2004) J Chromatogr A 1026:19–23

    Article  CAS  Google Scholar 

  15. Breithaupt DE (2004) Food Chem 86:449–456

    Article  CAS  Google Scholar 

  16. Dunford NT, Zhang M (2003) Food Res Int 36:905–909

    Article  CAS  Google Scholar 

  17. Herrero M, Ibáñez E, Señorans FJ, Cifuentes A (2004) J Chromatogr A 1047:195–203

    CAS  Google Scholar 

  18. Cavero S, Jaime L, Martín-Álvarez PJ, Señorans FJ, Reglero G, Ibáñez E (2005) Eur Food Res Technol 221:478–486

    Article  CAS  Google Scholar 

  19. Brand-Willians W, Cuvelier ME, Berset C (1995) Lebensm Wiss Technol 28:25–30

    Google Scholar 

  20. Jaime L, Mendiola JA, Herrero M, Soler-Rivas C, Santoyo S, Señorans FJ, Cifuentes A, Ibáñez E (2005) J Sep Sci 28:2111–2119

    Article  CAS  Google Scholar 

  21. NCCLS (National Committee for Clinical Laboratory Standards) (1999) Performance standards for antimicrobial susceptibility testing, 9th edn. International Supplement, M100-S9, Wayne, PA

  22. Frankel EN (1979) Analytical methods used in the study of autoxidation processes. In: Simic MG, Karel M (eds) Autoxidation in food and biological systems. Plenum Press, New York, pp 141–170

    Google Scholar 

  23. Singh RP, Chidambara KN, Jayaprakasha GK (2002) J Agric Food Chem 50:81–86

    Article  CAS  Google Scholar 

  24. Herrero H, Martín-Álvarez PJ, Señoráns FJ, Cifuentes A, Ibáñez E (2005) Food Chem 93:417–423

    Article  CAS  Google Scholar 

  25. Van den Hoek C, Mann DG, Jahns HM (1995) Algae: an introduction to phycology. Cambridge University Press, Cambridge, UK

    Google Scholar 

  26. Gireesh T, Jayadeep A, Rajasekharan KN, Menon VP, Vairamany M, Tang G, Nair PP, Sudhakaran PR (2001) Biotechnol Lett 23:447–449

    Article  CAS  Google Scholar 

  27. Quach HT, Steeper RL, Griffin GW (2004) J Chem Educ 81:385–387

    Article  CAS  Google Scholar 

  28. Soler-Rivas C, Espín JC, Wichers HJ (2000) Phytochem Anal 11:330–338

    Article  CAS  Google Scholar 

  29. Borowitzka MA (1995) J Appl Phycol 7:3–15

    Article  CAS  Google Scholar 

  30. Kreitlow S, Mundt S, Lindequist U (1999) J Biotechnol 70:61–63

    Article  CAS  Google Scholar 

  31. Demule MCZ, Decaire GZ, Decano MS (1996) J Exp Bot 58:93–96

    Google Scholar 

  32. Xue C, Hu Y, Saito H, Zhang Z, Li Z, Cai Y, Ou C, Lin H, Imbs AB (2002) Food Chem 77:9–13

    Article  CAS  Google Scholar 

Download references

Acknowledgements

MH thanks Ministerio de Ciencia y Tecnología for a FPI grant. Authors thank Ministerio de Ciencia y Tecnología (project AGL2002-04621-C02-02) for financial support.

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Authors and Affiliations

  1. Sección Departamental Ciencias de la Alimentación, Facultad de Ciencias, Módulo C-XVI, 505, Universidad Autónoma de Madrid, Ciudad Universitaria de Cantoblanco, Ctra. Colmenar Km 15, 28049, Madrid, Spain

    Susana Santoyo, F. Javier Señorans & Laura Jaime

  2. Instituto de Fermentaciones Industriales, CSIC, Juan de la Cierva 3, 28006, Madrid, Spain

    Miguel Herrero, Alejandro Cifuentes & Elena Ibáñez

Authors
  1. Susana Santoyo
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  2. Miguel Herrero
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  3. F. Javier Señorans
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  4. Alejandro Cifuentes
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  5. Elena Ibáñez
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  6. Laura Jaime
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Correspondence to Laura Jaime.

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Santoyo, S., Herrero, M., Señorans, F.J. et al. Functional characterization of pressurized liquid extracts of Spirulina platensis . Eur Food Res Technol 224, 75–81 (2006). https://doi.org/10.1007/s00217-006-0291-3

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  • DOI: https://doi.org/10.1007/s00217-006-0291-3

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